Strongyloidiasis, a parasitic disease of humans caused by the Strongyloides nematode, was recently added to the list of neglected tropical diseases requiring control measures in endemic areas(1) . According to the recent estimates, 614 million people are infected worldwide(2) . Metagenomic analysis of model parasite multihost systems is a key to uncovering the molecular diversity required to understand disease transmission. In our project, we confirmed the transmission of zoonotic Strongyloides fuelleborni haplotype between lowland gorillas and humans. In addition, we have detected Strongyloides stercoralis in both dogs and humans.